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ImmunoStar inc rabbit anti-vip antibody
Rabbit Anti Vip Antibody, supplied by ImmunoStar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vip+(rabbit)+antibody/anti+vip/pmc12208536-113-36-42
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rabbit anti-vip antibody - by Bioz Stars, 2026-09
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Incubation:

Article Title: Heterogeneous plasticity of amygdala interneurons in associative learning and extinction
Article Snippet: Slices were subsequently incubated in a combination of the following primary antibodies in carrier solution (1% NHS, 0.5% Triton X-100 in PBS) for 48 h at 4 °C: rabbit anti-VIP (1:1000, Immunostar, 20077, LOT# 1339001), rat anti-SST (1:500, Merck Millipore, MAB354, LOT# 232625), guinea pig anti-PV (1:500, Synaptic Systems, 195004, LOT# 195004/10).

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP cell visualization, 50 μm thick brain tissue sections that contained the SCN were first processed in 10% and 20% sucrose solution for 30 minutes each, and after a freeze-thaw step, they were incubated with rabbit anti-VIP antibody (1:1,000; catalog 20077, ImmunoStar) for 72 hours at 4°C.

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP neuron labeling, sections were incubated with rabbit anti-VIP antibody (1:500; catalog 20077, ImmunoStar) for 72 hours at 4°C and then with donkey anti–rabbit 647 (1:500; catalog A31573 , Thermo Fisher Scientific) for 2 hours at room temperature.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study.
Article Snippet: Four additional 50 μm sections were double-immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300-109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene– related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth-associated protein 43 (Rabbit GAP-43, 1:1000, Cat# NB300-143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma-Aldrich).26–28 The secondary antibodies used were anti-mouse Alexa Fluor 594 (1:600, Cat# ZF-0513, ZSGB-BIO) and anti-rabbit Alexa Fluor 488 (1:800, Cat# ZF-0511, ZSGB-BIO).

Article Title: Postnatal development of vasoactive intestinal polypeptide-expressing GABAergic interneurons in mouse somatosensory cortex.
Article Snippet: The sections were incubated for 3 days at 4°C in the primary antibodies (ABs) rabbitanti- VIP (1:2000, #20077, ImmunoStar, Dietzenbach, Germany) and goat- anti- mCherry (1:100, AB0040, Sicgen, Cantanhede, Portugal) in a staining buffer containing 2% bovine serum albumin (001- 000- 161, Dianova) with 0.05% azide and 0.3% Triton in 0.01 M PBS.

Article Title: Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates.
Article Snippet: While the enteric nervous system (ENS) of jawed vertebrates is largely derived from the vagal neural crest, lamprey are jawless vertebrates that lack the vagal neural crest, yet possess enteric neurons derived from latemigrating Schwann cell precursors.. To illuminate homologies between the ENS of jawed and jawless vertebrates, here we examine the diversity and distribution of neuronal subtypes within the intestine of the sea lamprey during late embryonic and ammocete stages.. In addition to previously described 5-HT-immunoreactive serotonergic neurons, we identified NOS and VIP neurons, consistent with motor neuron identity.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study
Article Snippet: Four additional 50 μm sections were double‐immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300‐109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene–related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth‐associated protein 43 (Rabbit GAP‐43, 1:1000, Cat# NB300‐143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma‐Aldrich)., , The secondary antibodies used were anti‐mouse Alexa Fluor 594 (1:600, Cat# ZF‐0513, ZSGB‐BIO) and anti‐rabbit Alexa Fluor 488 (1:800, Cat# ZF‐0511, ZSGB‐BIO).

Article Title: Stimulation of an entorhinal-hippocampal extinction circuit facilitates fear extinction in a post-traumatic stress disorder model
Article Snippet: Slices were incubated in the blocking solution with mouse anti-PV (1:300, Sigma-Aldrich, catalog no. MAB1572), goat anti-somatostatin (SST) (1:500, Santa Cruz, catalog no. sc-7819), rabbit anti-VIP (1:500, Immunostar, catalog no. 20077) or rabbit anti-Reelin (1:500, Invitrogen, catalog no. PA5-78413) at 4 °C overnight and then with the secondary antibody (Alexa Fluor 568 donkey anti-mouse IgG, Alexa Fluor 488 donkey anti-mouse IgG, Alexa Fluor 647 donkey anti-mouse IgG, Alexa Fluor 568 donkey anti-goat IgG, Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h. Slices were washed in 1 × PBS with 0.1% Tween-20, mounted onto slides, and covered with coverslips with ProLong Gold Antifade Mountant (Invitrogen).

Labeling:

Article Title: Heterogeneous plasticity of amygdala interneurons in associative learning and extinction
Article Snippet: Slices were subsequently incubated in a combination of the following primary antibodies in carrier solution (1% NHS, 0.5% Triton X-100 in PBS) for 48 h at 4 °C: rabbit anti-VIP (1:1000, Immunostar, 20077, LOT# 1339001), rat anti-SST (1:500, Merck Millipore, MAB354, LOT# 232625), guinea pig anti-PV (1:500, Synaptic Systems, 195004, LOT# 195004/10).

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP cell visualization, 50 μm thick brain tissue sections that contained the SCN were first processed in 10% and 20% sucrose solution for 30 minutes each, and after a freeze-thaw step, they were incubated with rabbit anti-VIP antibody (1:1,000; catalog 20077, ImmunoStar) for 72 hours at 4°C.

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP neuron labeling, sections were incubated with rabbit anti-VIP antibody (1:500; catalog 20077, ImmunoStar) for 72 hours at 4°C and then with donkey anti–rabbit 647 (1:500; catalog A31573 , Thermo Fisher Scientific) for 2 hours at room temperature.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study.
Article Snippet: Four additional 50 μm sections were double-immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300-109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene– related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth-associated protein 43 (Rabbit GAP-43, 1:1000, Cat# NB300-143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma-Aldrich).26–28 The secondary antibodies used were anti-mouse Alexa Fluor 594 (1:600, Cat# ZF-0513, ZSGB-BIO) and anti-rabbit Alexa Fluor 488 (1:800, Cat# ZF-0511, ZSGB-BIO).

Article Title: Postnatal development of vasoactive intestinal polypeptide-expressing GABAergic interneurons in mouse somatosensory cortex.
Article Snippet: The sections were incubated for 3 days at 4°C in the primary antibodies (ABs) rabbitanti- VIP (1:2000, #20077, ImmunoStar, Dietzenbach, Germany) and goat- anti- mCherry (1:100, AB0040, Sicgen, Cantanhede, Portugal) in a staining buffer containing 2% bovine serum albumin (001- 000- 161, Dianova) with 0.05% azide and 0.3% Triton in 0.01 M PBS.

Article Title: Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates.
Article Snippet: While the enteric nervous system (ENS) of jawed vertebrates is largely derived from the vagal neural crest, lamprey are jawless vertebrates that lack the vagal neural crest, yet possess enteric neurons derived from latemigrating Schwann cell precursors.. To illuminate homologies between the ENS of jawed and jawless vertebrates, here we examine the diversity and distribution of neuronal subtypes within the intestine of the sea lamprey during late embryonic and ammocete stages.. In addition to previously described 5-HT-immunoreactive serotonergic neurons, we identified NOS and VIP neurons, consistent with motor neuron identity.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study
Article Snippet: Four additional 50 μm sections were double‐immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300‐109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene–related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth‐associated protein 43 (Rabbit GAP‐43, 1:1000, Cat# NB300‐143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma‐Aldrich)., , The secondary antibodies used were anti‐mouse Alexa Fluor 594 (1:600, Cat# ZF‐0513, ZSGB‐BIO) and anti‐rabbit Alexa Fluor 488 (1:800, Cat# ZF‐0511, ZSGB‐BIO).

Article Title: Stimulation of an entorhinal-hippocampal extinction circuit facilitates fear extinction in a post-traumatic stress disorder model
Article Snippet: Slices were incubated in the blocking solution with mouse anti-PV (1:300, Sigma-Aldrich, catalog no. MAB1572), goat anti-somatostatin (SST) (1:500, Santa Cruz, catalog no. sc-7819), rabbit anti-VIP (1:500, Immunostar, catalog no. 20077) or rabbit anti-Reelin (1:500, Invitrogen, catalog no. PA5-78413) at 4 °C overnight and then with the secondary antibody (Alexa Fluor 568 donkey anti-mouse IgG, Alexa Fluor 488 donkey anti-mouse IgG, Alexa Fluor 647 donkey anti-mouse IgG, Alexa Fluor 568 donkey anti-goat IgG, Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h. Slices were washed in 1 × PBS with 0.1% Tween-20, mounted onto slides, and covered with coverslips with ProLong Gold Antifade Mountant (Invitrogen).

Marker:

Article Title: Heterogeneous plasticity of amygdala interneurons in associative learning and extinction
Article Snippet: Slices were subsequently incubated in a combination of the following primary antibodies in carrier solution (1% NHS, 0.5% Triton X-100 in PBS) for 48 h at 4 °C: rabbit anti-VIP (1:1000, Immunostar, 20077, LOT# 1339001), rat anti-SST (1:500, Merck Millipore, MAB354, LOT# 232625), guinea pig anti-PV (1:500, Synaptic Systems, 195004, LOT# 195004/10).

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP cell visualization, 50 μm thick brain tissue sections that contained the SCN were first processed in 10% and 20% sucrose solution for 30 minutes each, and after a freeze-thaw step, they were incubated with rabbit anti-VIP antibody (1:1,000; catalog 20077, ImmunoStar) for 72 hours at 4°C.

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP neuron labeling, sections were incubated with rabbit anti-VIP antibody (1:500; catalog 20077, ImmunoStar) for 72 hours at 4°C and then with donkey anti–rabbit 647 (1:500; catalog A31573 , Thermo Fisher Scientific) for 2 hours at room temperature.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study.
Article Snippet: Four additional 50 μm sections were double-immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300-109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene– related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth-associated protein 43 (Rabbit GAP-43, 1:1000, Cat# NB300-143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma-Aldrich).26–28 The secondary antibodies used were anti-mouse Alexa Fluor 594 (1:600, Cat# ZF-0513, ZSGB-BIO) and anti-rabbit Alexa Fluor 488 (1:800, Cat# ZF-0511, ZSGB-BIO).

Article Title: Postnatal development of vasoactive intestinal polypeptide-expressing GABAergic interneurons in mouse somatosensory cortex.
Article Snippet: The sections were incubated for 3 days at 4°C in the primary antibodies (ABs) rabbitanti- VIP (1:2000, #20077, ImmunoStar, Dietzenbach, Germany) and goat- anti- mCherry (1:100, AB0040, Sicgen, Cantanhede, Portugal) in a staining buffer containing 2% bovine serum albumin (001- 000- 161, Dianova) with 0.05% azide and 0.3% Triton in 0.01 M PBS.

Article Title: Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates.
Article Snippet: While the enteric nervous system (ENS) of jawed vertebrates is largely derived from the vagal neural crest, lamprey are jawless vertebrates that lack the vagal neural crest, yet possess enteric neurons derived from latemigrating Schwann cell precursors.. To illuminate homologies between the ENS of jawed and jawless vertebrates, here we examine the diversity and distribution of neuronal subtypes within the intestine of the sea lamprey during late embryonic and ammocete stages.. In addition to previously described 5-HT-immunoreactive serotonergic neurons, we identified NOS and VIP neurons, consistent with motor neuron identity.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study
Article Snippet: Four additional 50 μm sections were double‐immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300‐109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene–related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth‐associated protein 43 (Rabbit GAP‐43, 1:1000, Cat# NB300‐143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma‐Aldrich)., , The secondary antibodies used were anti‐mouse Alexa Fluor 594 (1:600, Cat# ZF‐0513, ZSGB‐BIO) and anti‐rabbit Alexa Fluor 488 (1:800, Cat# ZF‐0511, ZSGB‐BIO).

Article Title: Stimulation of an entorhinal-hippocampal extinction circuit facilitates fear extinction in a post-traumatic stress disorder model
Article Snippet: Slices were incubated in the blocking solution with mouse anti-PV (1:300, Sigma-Aldrich, catalog no. MAB1572), goat anti-somatostatin (SST) (1:500, Santa Cruz, catalog no. sc-7819), rabbit anti-VIP (1:500, Immunostar, catalog no. 20077) or rabbit anti-Reelin (1:500, Invitrogen, catalog no. PA5-78413) at 4 °C overnight and then with the secondary antibody (Alexa Fluor 568 donkey anti-mouse IgG, Alexa Fluor 488 donkey anti-mouse IgG, Alexa Fluor 647 donkey anti-mouse IgG, Alexa Fluor 568 donkey anti-goat IgG, Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h. Slices were washed in 1 × PBS with 0.1% Tween-20, mounted onto slides, and covered with coverslips with ProLong Gold Antifade Mountant (Invitrogen).

Confocal Microscopy:

Article Title: Heterogeneous plasticity of amygdala interneurons in associative learning and extinction
Article Snippet: Slices were subsequently incubated in a combination of the following primary antibodies in carrier solution (1% NHS, 0.5% Triton X-100 in PBS) for 48 h at 4 °C: rabbit anti-VIP (1:1000, Immunostar, 20077, LOT# 1339001), rat anti-SST (1:500, Merck Millipore, MAB354, LOT# 232625), guinea pig anti-PV (1:500, Synaptic Systems, 195004, LOT# 195004/10).

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP cell visualization, 50 μm thick brain tissue sections that contained the SCN were first processed in 10% and 20% sucrose solution for 30 minutes each, and after a freeze-thaw step, they were incubated with rabbit anti-VIP antibody (1:1,000; catalog 20077, ImmunoStar) for 72 hours at 4°C.

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP neuron labeling, sections were incubated with rabbit anti-VIP antibody (1:500; catalog 20077, ImmunoStar) for 72 hours at 4°C and then with donkey anti–rabbit 647 (1:500; catalog A31573 , Thermo Fisher Scientific) for 2 hours at room temperature.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study.
Article Snippet: Four additional 50 μm sections were double-immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300-109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene– related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth-associated protein 43 (Rabbit GAP-43, 1:1000, Cat# NB300-143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma-Aldrich).26–28 The secondary antibodies used were anti-mouse Alexa Fluor 594 (1:600, Cat# ZF-0513, ZSGB-BIO) and anti-rabbit Alexa Fluor 488 (1:800, Cat# ZF-0511, ZSGB-BIO).

Article Title: Postnatal development of vasoactive intestinal polypeptide-expressing GABAergic interneurons in mouse somatosensory cortex.
Article Snippet: The sections were incubated for 3 days at 4°C in the primary antibodies (ABs) rabbitanti- VIP (1:2000, #20077, ImmunoStar, Dietzenbach, Germany) and goat- anti- mCherry (1:100, AB0040, Sicgen, Cantanhede, Portugal) in a staining buffer containing 2% bovine serum albumin (001- 000- 161, Dianova) with 0.05% azide and 0.3% Triton in 0.01 M PBS.

Article Title: Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates.
Article Snippet: While the enteric nervous system (ENS) of jawed vertebrates is largely derived from the vagal neural crest, lamprey are jawless vertebrates that lack the vagal neural crest, yet possess enteric neurons derived from latemigrating Schwann cell precursors.. To illuminate homologies between the ENS of jawed and jawless vertebrates, here we examine the diversity and distribution of neuronal subtypes within the intestine of the sea lamprey during late embryonic and ammocete stages.. In addition to previously described 5-HT-immunoreactive serotonergic neurons, we identified NOS and VIP neurons, consistent with motor neuron identity.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study
Article Snippet: Four additional 50 μm sections were double‐immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300‐109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene–related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth‐associated protein 43 (Rabbit GAP‐43, 1:1000, Cat# NB300‐143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma‐Aldrich)., , The secondary antibodies used were anti‐mouse Alexa Fluor 594 (1:600, Cat# ZF‐0513, ZSGB‐BIO) and anti‐rabbit Alexa Fluor 488 (1:800, Cat# ZF‐0511, ZSGB‐BIO).

Article Title: Stimulation of an entorhinal-hippocampal extinction circuit facilitates fear extinction in a post-traumatic stress disorder model
Article Snippet: Slices were incubated in the blocking solution with mouse anti-PV (1:300, Sigma-Aldrich, catalog no. MAB1572), goat anti-somatostatin (SST) (1:500, Santa Cruz, catalog no. sc-7819), rabbit anti-VIP (1:500, Immunostar, catalog no. 20077) or rabbit anti-Reelin (1:500, Invitrogen, catalog no. PA5-78413) at 4 °C overnight and then with the secondary antibody (Alexa Fluor 568 donkey anti-mouse IgG, Alexa Fluor 488 donkey anti-mouse IgG, Alexa Fluor 647 donkey anti-mouse IgG, Alexa Fluor 568 donkey anti-goat IgG, Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h. Slices were washed in 1 × PBS with 0.1% Tween-20, mounted onto slides, and covered with coverslips with ProLong Gold Antifade Mountant (Invitrogen).

Control:

Article Title: Heterogeneous plasticity of amygdala interneurons in associative learning and extinction
Article Snippet: Slices were subsequently incubated in a combination of the following primary antibodies in carrier solution (1% NHS, 0.5% Triton X-100 in PBS) for 48 h at 4 °C: rabbit anti-VIP (1:1000, Immunostar, 20077, LOT# 1339001), rat anti-SST (1:500, Merck Millipore, MAB354, LOT# 232625), guinea pig anti-PV (1:500, Synaptic Systems, 195004, LOT# 195004/10).

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP cell visualization, 50 μm thick brain tissue sections that contained the SCN were first processed in 10% and 20% sucrose solution for 30 minutes each, and after a freeze-thaw step, they were incubated with rabbit anti-VIP antibody (1:1,000; catalog 20077, ImmunoStar) for 72 hours at 4°C.

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP neuron labeling, sections were incubated with rabbit anti-VIP antibody (1:500; catalog 20077, ImmunoStar) for 72 hours at 4°C and then with donkey anti–rabbit 647 (1:500; catalog A31573 , Thermo Fisher Scientific) for 2 hours at room temperature.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study.
Article Snippet: Four additional 50 μm sections were double-immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300-109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene– related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth-associated protein 43 (Rabbit GAP-43, 1:1000, Cat# NB300-143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma-Aldrich).26–28 The secondary antibodies used were anti-mouse Alexa Fluor 594 (1:600, Cat# ZF-0513, ZSGB-BIO) and anti-rabbit Alexa Fluor 488 (1:800, Cat# ZF-0511, ZSGB-BIO).

Article Title: Postnatal development of vasoactive intestinal polypeptide-expressing GABAergic interneurons in mouse somatosensory cortex.
Article Snippet: The sections were incubated for 3 days at 4°C in the primary antibodies (ABs) rabbitanti- VIP (1:2000, #20077, ImmunoStar, Dietzenbach, Germany) and goat- anti- mCherry (1:100, AB0040, Sicgen, Cantanhede, Portugal) in a staining buffer containing 2% bovine serum albumin (001- 000- 161, Dianova) with 0.05% azide and 0.3% Triton in 0.01 M PBS.

Article Title: Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates.
Article Snippet: While the enteric nervous system (ENS) of jawed vertebrates is largely derived from the vagal neural crest, lamprey are jawless vertebrates that lack the vagal neural crest, yet possess enteric neurons derived from latemigrating Schwann cell precursors.. To illuminate homologies between the ENS of jawed and jawless vertebrates, here we examine the diversity and distribution of neuronal subtypes within the intestine of the sea lamprey during late embryonic and ammocete stages.. In addition to previously described 5-HT-immunoreactive serotonergic neurons, we identified NOS and VIP neurons, consistent with motor neuron identity.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study
Article Snippet: Four additional 50 μm sections were double‐immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300‐109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene–related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth‐associated protein 43 (Rabbit GAP‐43, 1:1000, Cat# NB300‐143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma‐Aldrich)., , The secondary antibodies used were anti‐mouse Alexa Fluor 594 (1:600, Cat# ZF‐0513, ZSGB‐BIO) and anti‐rabbit Alexa Fluor 488 (1:800, Cat# ZF‐0511, ZSGB‐BIO).

Article Title: Stimulation of an entorhinal-hippocampal extinction circuit facilitates fear extinction in a post-traumatic stress disorder model
Article Snippet: Slices were incubated in the blocking solution with mouse anti-PV (1:300, Sigma-Aldrich, catalog no. MAB1572), goat anti-somatostatin (SST) (1:500, Santa Cruz, catalog no. sc-7819), rabbit anti-VIP (1:500, Immunostar, catalog no. 20077) or rabbit anti-Reelin (1:500, Invitrogen, catalog no. PA5-78413) at 4 °C overnight and then with the secondary antibody (Alexa Fluor 568 donkey anti-mouse IgG, Alexa Fluor 488 donkey anti-mouse IgG, Alexa Fluor 647 donkey anti-mouse IgG, Alexa Fluor 568 donkey anti-goat IgG, Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h. Slices were washed in 1 × PBS with 0.1% Tween-20, mounted onto slides, and covered with coverslips with ProLong Gold Antifade Mountant (Invitrogen).

Staining:

Article Title: Heterogeneous plasticity of amygdala interneurons in associative learning and extinction
Article Snippet: Slices were subsequently incubated in a combination of the following primary antibodies in carrier solution (1% NHS, 0.5% Triton X-100 in PBS) for 48 h at 4 °C: rabbit anti-VIP (1:1000, Immunostar, 20077, LOT# 1339001), rat anti-SST (1:500, Merck Millipore, MAB354, LOT# 232625), guinea pig anti-PV (1:500, Synaptic Systems, 195004, LOT# 195004/10).

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP cell visualization, 50 μm thick brain tissue sections that contained the SCN were first processed in 10% and 20% sucrose solution for 30 minutes each, and after a freeze-thaw step, they were incubated with rabbit anti-VIP antibody (1:1,000; catalog 20077, ImmunoStar) for 72 hours at 4°C.

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP neuron labeling, sections were incubated with rabbit anti-VIP antibody (1:500; catalog 20077, ImmunoStar) for 72 hours at 4°C and then with donkey anti–rabbit 647 (1:500; catalog A31573 , Thermo Fisher Scientific) for 2 hours at room temperature.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study.
Article Snippet: Four additional 50 μm sections were double-immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300-109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene– related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth-associated protein 43 (Rabbit GAP-43, 1:1000, Cat# NB300-143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma-Aldrich).26–28 The secondary antibodies used were anti-mouse Alexa Fluor 594 (1:600, Cat# ZF-0513, ZSGB-BIO) and anti-rabbit Alexa Fluor 488 (1:800, Cat# ZF-0511, ZSGB-BIO).

Article Title: Postnatal development of vasoactive intestinal polypeptide-expressing GABAergic interneurons in mouse somatosensory cortex.
Article Snippet: The sections were incubated for 3 days at 4°C in the primary antibodies (ABs) rabbitanti- VIP (1:2000, #20077, ImmunoStar, Dietzenbach, Germany) and goat- anti- mCherry (1:100, AB0040, Sicgen, Cantanhede, Portugal) in a staining buffer containing 2% bovine serum albumin (001- 000- 161, Dianova) with 0.05% azide and 0.3% Triton in 0.01 M PBS.

Article Title: Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates.
Article Snippet: While the enteric nervous system (ENS) of jawed vertebrates is largely derived from the vagal neural crest, lamprey are jawless vertebrates that lack the vagal neural crest, yet possess enteric neurons derived from latemigrating Schwann cell precursors.. To illuminate homologies between the ENS of jawed and jawless vertebrates, here we examine the diversity and distribution of neuronal subtypes within the intestine of the sea lamprey during late embryonic and ammocete stages.. In addition to previously described 5-HT-immunoreactive serotonergic neurons, we identified NOS and VIP neurons, consistent with motor neuron identity.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study
Article Snippet: Four additional 50 μm sections were double‐immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300‐109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene–related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth‐associated protein 43 (Rabbit GAP‐43, 1:1000, Cat# NB300‐143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma‐Aldrich)., , The secondary antibodies used were anti‐mouse Alexa Fluor 594 (1:600, Cat# ZF‐0513, ZSGB‐BIO) and anti‐rabbit Alexa Fluor 488 (1:800, Cat# ZF‐0511, ZSGB‐BIO).

Article Title: Stimulation of an entorhinal-hippocampal extinction circuit facilitates fear extinction in a post-traumatic stress disorder model
Article Snippet: Slices were incubated in the blocking solution with mouse anti-PV (1:300, Sigma-Aldrich, catalog no. MAB1572), goat anti-somatostatin (SST) (1:500, Santa Cruz, catalog no. sc-7819), rabbit anti-VIP (1:500, Immunostar, catalog no. 20077) or rabbit anti-Reelin (1:500, Invitrogen, catalog no. PA5-78413) at 4 °C overnight and then with the secondary antibody (Alexa Fluor 568 donkey anti-mouse IgG, Alexa Fluor 488 donkey anti-mouse IgG, Alexa Fluor 647 donkey anti-mouse IgG, Alexa Fluor 568 donkey anti-goat IgG, Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h. Slices were washed in 1 × PBS with 0.1% Tween-20, mounted onto slides, and covered with coverslips with ProLong Gold Antifade Mountant (Invitrogen).

Blocking Assay:

Article Title: Heterogeneous plasticity of amygdala interneurons in associative learning and extinction
Article Snippet: Slices were subsequently incubated in a combination of the following primary antibodies in carrier solution (1% NHS, 0.5% Triton X-100 in PBS) for 48 h at 4 °C: rabbit anti-VIP (1:1000, Immunostar, 20077, LOT# 1339001), rat anti-SST (1:500, Merck Millipore, MAB354, LOT# 232625), guinea pig anti-PV (1:500, Synaptic Systems, 195004, LOT# 195004/10).

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP cell visualization, 50 μm thick brain tissue sections that contained the SCN were first processed in 10% and 20% sucrose solution for 30 minutes each, and after a freeze-thaw step, they were incubated with rabbit anti-VIP antibody (1:1,000; catalog 20077, ImmunoStar) for 72 hours at 4°C.

Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
Article Snippet: For VIP neuron labeling, sections were incubated with rabbit anti-VIP antibody (1:500; catalog 20077, ImmunoStar) for 72 hours at 4°C and then with donkey anti–rabbit 647 (1:500; catalog A31573 , Thermo Fisher Scientific) for 2 hours at room temperature.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study.
Article Snippet: Four additional 50 μm sections were double-immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300-109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene– related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth-associated protein 43 (Rabbit GAP-43, 1:1000, Cat# NB300-143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma-Aldrich).26–28 The secondary antibodies used were anti-mouse Alexa Fluor 594 (1:600, Cat# ZF-0513, ZSGB-BIO) and anti-rabbit Alexa Fluor 488 (1:800, Cat# ZF-0511, ZSGB-BIO).

Article Title: Postnatal development of vasoactive intestinal polypeptide-expressing GABAergic interneurons in mouse somatosensory cortex.
Article Snippet: The sections were incubated for 3 days at 4°C in the primary antibodies (ABs) rabbitanti- VIP (1:2000, #20077, ImmunoStar, Dietzenbach, Germany) and goat- anti- mCherry (1:100, AB0040, Sicgen, Cantanhede, Portugal) in a staining buffer containing 2% bovine serum albumin (001- 000- 161, Dianova) with 0.05% azide and 0.3% Triton in 0.01 M PBS.

Article Title: Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates.
Article Snippet: While the enteric nervous system (ENS) of jawed vertebrates is largely derived from the vagal neural crest, lamprey are jawless vertebrates that lack the vagal neural crest, yet possess enteric neurons derived from latemigrating Schwann cell precursors.. To illuminate homologies between the ENS of jawed and jawless vertebrates, here we examine the diversity and distribution of neuronal subtypes within the intestine of the sea lamprey during late embryonic and ammocete stages.. In addition to previously described 5-HT-immunoreactive serotonergic neurons, we identified NOS and VIP neurons, consistent with motor neuron identity.

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study
Article Snippet: Four additional 50 μm sections were double‐immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300‐109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene–related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth‐associated protein 43 (Rabbit GAP‐43, 1:1000, Cat# NB300‐143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma‐Aldrich)., , The secondary antibodies used were anti‐mouse Alexa Fluor 594 (1:600, Cat# ZF‐0513, ZSGB‐BIO) and anti‐rabbit Alexa Fluor 488 (1:800, Cat# ZF‐0511, ZSGB‐BIO).

Article Title: Stimulation of an entorhinal-hippocampal extinction circuit facilitates fear extinction in a post-traumatic stress disorder model
Article Snippet: Slices were incubated in the blocking solution with mouse anti-PV (1:300, Sigma-Aldrich, catalog no. MAB1572), goat anti-somatostatin (SST) (1:500, Santa Cruz, catalog no. sc-7819), rabbit anti-VIP (1:500, Immunostar, catalog no. 20077) or rabbit anti-Reelin (1:500, Invitrogen, catalog no. PA5-78413) at 4 °C overnight and then with the secondary antibody (Alexa Fluor 568 donkey anti-mouse IgG, Alexa Fluor 488 donkey anti-mouse IgG, Alexa Fluor 647 donkey anti-mouse IgG, Alexa Fluor 568 donkey anti-goat IgG, Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h. Slices were washed in 1 × PBS with 0.1% Tween-20, mounted onto slides, and covered with coverslips with ProLong Gold Antifade Mountant (Invitrogen).



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Noradrenergic, cholinergic, peptidergic, and regenerative nerve fibers in patients with NIID and HCs. Confocal microscopy images of skin sections paired with corresponding quantitative analyses revealing a comparative investigation of different nerve fibers in patients with NIID and HCs, including TH‐labeled noradrenergic nerve fibers (A–C), VIP‐labeled cholinergic nerve fibers (D–F), CGRP (G–I)‐ and SP (J–L)‐labeled peptidergic nerve fibers, and GAP‐43‐labeled somatosensory (M–O) and autonomic regenerative nerve fibers (P–R). Scale bars = 50 µm. ***, p < 0.001. CGRP, calcitonin gene–related peptide; GAP‐43, growth‐associated protein 43; HC, healthy control; NIID, neuronal intranuclear inclusion disease; SP, substance P; TH, tyrosine hydroxylase; VIP, vasoactive <t>intestinal</t> peptide.
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Image Search Results


Noradrenergic, cholinergic, peptidergic, and regenerative nerve fibers in patients with NIID and HCs. Confocal microscopy images of skin sections paired with corresponding quantitative analyses revealing a comparative investigation of different nerve fibers in patients with NIID and HCs, including TH‐labeled noradrenergic nerve fibers (A–C), VIP‐labeled cholinergic nerve fibers (D–F), CGRP (G–I)‐ and SP (J–L)‐labeled peptidergic nerve fibers, and GAP‐43‐labeled somatosensory (M–O) and autonomic regenerative nerve fibers (P–R). Scale bars = 50 µm. ***, p < 0.001. CGRP, calcitonin gene–related peptide; GAP‐43, growth‐associated protein 43; HC, healthy control; NIID, neuronal intranuclear inclusion disease; SP, substance P; TH, tyrosine hydroxylase; VIP, vasoactive intestinal peptide.

Journal: Alzheimer's & Dementia

Article Title: Identification of small fiber neuropathy in neuronal intranuclear inclusion disease: A clinicopathological study

doi: 10.1002/alz.14596

Figure Lengend Snippet: Noradrenergic, cholinergic, peptidergic, and regenerative nerve fibers in patients with NIID and HCs. Confocal microscopy images of skin sections paired with corresponding quantitative analyses revealing a comparative investigation of different nerve fibers in patients with NIID and HCs, including TH‐labeled noradrenergic nerve fibers (A–C), VIP‐labeled cholinergic nerve fibers (D–F), CGRP (G–I)‐ and SP (J–L)‐labeled peptidergic nerve fibers, and GAP‐43‐labeled somatosensory (M–O) and autonomic regenerative nerve fibers (P–R). Scale bars = 50 µm. ***, p < 0.001. CGRP, calcitonin gene–related peptide; GAP‐43, growth‐associated protein 43; HC, healthy control; NIID, neuronal intranuclear inclusion disease; SP, substance P; TH, tyrosine hydroxylase; VIP, vasoactive intestinal peptide.

Article Snippet: Four additional 50 µm sections were double‐immunostained to detect different types of small nerve fibers using a large panel of antibodies, including primary antibodies against vasoactive intestinal peptide (Rabbit VIP, 1:200, Cat# 20077, ImmunoStar) as a marker of cholinergic fibers, tyrosine hydroxylase (Rabbit TH, 1:1000, Cat# NB300‐109, Novus Biologicals) as a marker of noradrenergic fibers, substance P (Rabbit SP, 1:1,000, Cat# 20064, ImmunoStar), and calcitonin gene–related peptide (Rabbit CGRP, 1:500, Cat# 24112, ImmunoStar) as markers of peptidergic nerve fibers, growth‐associated protein 43 (Rabbit GAP‐43, 1:1000, Cat# NB300‐143, Novus Biologicals) as a marker of regenerative fibers, and collagen type IV (Mouse ColIV, 1:800, Cat# MAB1910, Sigma‐Aldrich)., , The secondary antibodies used were anti‐mouse Alexa Fluor 594 (1:600, Cat# ZF‐0513, ZSGB‐BIO) and anti‐rabbit Alexa Fluor 488 (1:800, Cat# ZF‐0511, ZSGB‐BIO).

Techniques: Confocal Microscopy, Labeling, Control

List of primary and secondary antibodies used for immunostaining.

Journal: Scientific Reports

Article Title: Giant pyramidal neurons of the primary motor cortex express vasoactive intestinal polypeptide (VIP), a known marker of cortical interneurons

doi: 10.1038/s41598-024-71637-3

Figure Lengend Snippet: List of primary and secondary antibodies used for immunostaining.

Article Snippet: VIP-IF , Rabbit polyclonal (1:200) , Atlas antibodies Cat# HPA017324; RRID: AB_1858754 , Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (1:600) , Abcam Cat# ab150064; RRID: AB_2734146.

Techniques: Immunostaining, Plasmid Preparation, Marker